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What is liquid-liquid partitioning?



Liquid-liquid partitioning, also known as liquid-liquid extraction, is a method used to separate and purify compounds in a mixture. It is based on the principle that different compounds have different solubilities in different liquids. In liquid-liquid partitioning, a mixture of compounds is added to a separating funnel or a centrifugal partition chromatographer (CPC) containing two immiscible liquids, called the solvent and the extractant. The different compounds in the mixture will partition themselves between the two liquids based on their solubility, so that one liquid will contain more of certain compounds than the other. The two liquids can then be physically separated and the different compounds can be collected from each liquid. This process can be repeated multiple times to further purify the compounds.

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